The AlphaLISA® High Performance (HP) Human CXCL10 Detection Kit is designed for the quantitative determination of human CXCL10 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL259.
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C-X-C Motif Chemokine 10 (CXCL10), also known as 10 kDa Interferon-gamma-Induced protein (IP-10), is a 77 amino acid protein secreted by several cell types in response to IFNγ. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 holds several roles such as chemoattraction for monocytes, T-cells, and dendritic cells. Other activities include promotion of T-cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation. It has been suggested that CXCL10 plays an important role in delayed hypersensitivity reactions. Increased levels of CXCL10 are found in psoriatic plaques characterized by the infiltration of neutrophils, but it does not activate neutrophils. It was reported that CXCL10 also possesses antimicrobial activity. Recent data suggest that CXCL10 could bind to toll-like receptor 4 and may contribute to beta cell failure in diabetes.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
| Assay Points (200-50,000) | 100 Assay Points |
|---|---|
| Assay Target | CXCL10 |
| Assay Target Class | Cytokine |
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Therapeutic Area | Autoimmunity, Oncology, Immuno-oncology |
| Unit Size | 100 Assay Points |
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