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The AlphaLISA® High Performance (HP) Human CXCL10 Detection Kit is designed for the quantitative determination of human CXCL10 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL259.
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C-X-C Motif Chemokine 10 (CXCL10), also known as 10 kDa Interferon-gamma-Induced protein (IP-10), is a 77 amino acid protein secreted by several cell types in response to IFNγ. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 holds several roles such as chemoattraction for monocytes, T-cells, and dendritic cells. Other activities include promotion of T-cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation. It has been suggested that CXCL10 plays an important role in delayed hypersensitivity reactions. Increased levels of CXCL10 are found in psoriatic plaques characterized by the infiltration of neutrophils, but it does not activate neutrophils. It was reported that CXCL10 also possesses antimicrobial activity. Recent data suggest that CXCL10 could bind to toll-like receptor 4 and may contribute to beta cell failure in diabetes.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
| Assay Points (200-50,000) | 500 Assay Points |
|---|---|
| Assay Target | CXCL10 |
| Assay Target Class | Cytokine |
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA |
| Shipping Condition | Blue Ice |
| Target Species | Human |
| Therapeutic Area | Autoimmunity, Oncology, Immuno-oncology |
| Unit Size | 500 Assay Points |
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Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
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Utilize this guide ...
Find out about our range of integrated solutions for drug discovery screening in this e-brochure.
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Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on other cells. Identification of cytokines, their receptors and signaling pathways, and application of these novel observations to the clinic has made the development of ne ...
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
OptiPlate-384, White Opaque 384-well Microplate, Case 50
List Price : USD371.0
AlphaPlate-384, Light gray, untreated, Case of 50
List Price : USD330.0
List Price : USD85.0
