The AlphaLISA® High Performance (HP) Human IL-10 Detection Kit is designed for the quantitative determination of human IL-10 in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL218.
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Human Interleukin 10 (IL-10) is a homodimer composed of two subunits of 18 kDa each. It is produced by various T-cell populations, monocytes, macrophages, and different cell types in the liver when stimulated by endogenous or exogenous factors such as stress, exotoxins, TNF-α, and catecholamines. IL-10 inhibits interferon synthesis in Th1 cell clones, monocytes, and macrophages. IL-10 also inhibits antigen presentation to T-cells and IL-12 production by monocytes. It also impairs the proliferation and cytokine synthesis of CD4+ T cells, without having a direct inhibitory effect on CD8+ T cells. On the other hand, IL-10 has a stimulatory effect on B cells, prevents apoptosis and enhances proliferation and differentiation of plasma cells, and inhibits the release of various chemokines by neutrophils. The main biological function of IL-10 is to limit and terminate the inflammatory responses, block proinflammatory cytokine secretion and regulate the differentiation and proliferation of several immune cells. IL-10 activity is mediated by the heteromeric IL-10 receptor (IL-10R), and signals through the tyrosine kinases JAK1 and TYK2, and STATs.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
| 檢測點（200-50,000） | 100 Assay Points |
|---|---|
| 檢測目標 | IL-10 |
| 檢測目標類 | Cytokine |
| 檢測技術 | Alpha |
| 自動化兼容 | Yes |
| 檢測方法 | Alpha |
| 實驗類型 | In vitro |
| 產品品牌名稱 | AlphaLISA |
| 運輸條件 | 藍冰 |
| Target Species | Human |
| 治療領域 | Autoimmunity, Neuroscience, Cardiovascular, Oncology, Immuno-oncology |
| 產品尺寸 | 100 Assay Points |
Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run cytokine assays, it is key to consider a multitude of factors that impact the assay performance, such as sample dilutions and cell culture format.
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Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on other cells. Identification of cytokines, their receptors and signaling pathways, and application of these novel observations to the clinic has made the development of ne ...
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Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
