The AlphaLISA® High Performance (HP) Human IL-1β Detection Kit is designed for the quantitative determination of human IL-1β in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL220.
false falseFor research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Interleukin 1 beta (IL-1β) is a central player of the immune response, involved in inflammation at local and systemic levels. IL-1β is synthesized as a 31 kDa precursor that is cleaved by caspase-1 (ICE) into the active 17 kDa form and eventually released into extracellular space. Its production has been reported in many cell types including brain and monocytic and peripheral blood mononuclear cells. After binding to its receptor, IL-1RI, IL-1β triggers a cascade of kinase signaling pathways that lead to the activation of transcription factors like NFκB and AP-1, eventually activating the expression of genes such as MIP-2 and C-reactive protein.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
| 檢測點（200-50,000） | 100 Assay Points |
|---|---|
| 檢測目標 | IL-1β |
| 檢測目標類 | Cytokine |
| 檢測技術 | Alpha |
| 自動化兼容 | Yes |
| 檢測方法 | Alpha |
| 實驗類型 | In vitro |
| 產品品牌名稱 | AlphaLISA |
| 運輸條件 | 藍冰 |
| Target Species | Human |
| 治療領域 | Autoimmunity, Neuroscience, Oncology, Inflammation, Metabolic, Immuno-oncology |
| 產品尺寸 | 100 Assay Points |
Cytokines are implicated in the pathology and outcome of disease states across all therapeutic areas. When preparing to run cytokine assays, it is key to consider a multitude of factors that impact the assay performance, such as sample dilutions and cell culture format.
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Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
Cytokines play a vital role in both innate and adaptive immunity and are known for their ability to exert diverse functions on other cells. Identification of cytokines, their receptors and signaling pathways, and application of these novel observations to the clinic has made the development of ne ...
Cytokines play an essential role in inflammatory processes, leading to their involvement in viral and bacterial infections, as well as the pathology of many diseases including cancer, autoimmune conditions, neurodegenerative disorders, and atherosclerosis. As such, cytokines are often used eithe ...
Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology is a bead-based, no-wash alternative to traditional ELISAs. Instead of detection with an HRP substrate, the Alpha assay signal is generated by the excitation of an Eu+-coated bead that has been ...
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Biomarker levels were measured directly in CulturPlates-96 and-384 in a simple, fast, all-in-one-well AlphaLISA assay format. The elimination of transfer and wash steps simplifies cellular assays, reduces variability and significantly reduces hands-on time and costs associated with consumables. Int ...
The AlphaLISA® assay is a homogeneous immunoassay alternative to classical ELISA. AlphaLISA assays were originally utilized to detect analytesin cell cultures upernatants or serum/plasma samples.
