The AlphaLISA® High Performance (HP) Mouse IFNγ Detection Kit is designed for the quantitative determination of mouse IFNγ in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.
This kit is the new and improved version of part number AL501.
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Interferon gamma (IFNγ) is a homodimer glycosylated cytokine that is produced predominantly by natural killer and natural killer T cells. It is critical for innate and adaptive immunity against viral, bacterial, and protozoan infections, and is involved in tumor growth. In domestic animal species, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Genetic variation of this cytokine is also associated with the risk of aplastic anemia.
AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
| Assay Points (200-50,000) | 100 Assay Points |
|---|---|
| Assay Target | IFN-γ |
| Assay Target Class | Cytokine |
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA |
| Shipping Condition | Blue Ice |
| Target Species | Mouse |
| Therapeutic Area | Autoimmunity, Cardiovascular, Oncology, Inflammation, Immuno-oncology |
| Unit Size | 100 Assay Points |
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