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AlphaScreen® Rabbit IgG Detection Kit, 10,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-rabbit IgG conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
6760607C
500 assay points
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6760607M
10,000 assay points
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6760607R
50,000 assay points
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詳細信息

This bead kit can be used for many applications, including:

  • Antibody detection assays
  • Protein detection assays
  • Protein-protein interaction assays
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is an anti-species antibody, and associates with the corresponding AlphaScreen anti-species Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FITC probe provided in the kit.

規格

抗體偶聯物 Streptavidin/Anti-rabbit IgG
自動化兼容 Yes
產品品牌名稱 AlphaScreen
包裝量 10000.0 Units
運輸條件 藍冰
產品尺寸 10,000 assay points
資源
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產品手冊

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB

應用簡報

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

PDF 1 MB
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