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Alpha Glutathione Donor beads 1 mg

Alpha Donor beads coated with glutathione.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
6765300
1 mg
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6765301
5 mg
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6765302
25 mg
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詳細信息

These beads can be used to capture GST-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.

規格

抗體偶聯物 Glutathione
自動化兼容 Yes
珠型或核心珠型 Alpha Donor
產品品牌名稱 AlphaLISA
運輸條件 藍冰
產品尺寸 1 mg
資源
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產品手冊

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
Handle Large Biomolecular Interactions With Ease

The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measuremen ...

PDF 798 KB

技術說明

Development of an AlphaLISA Assay to Measure and Screen Inhibitors of the p53-MDM2 Interaction

Binding events between biomolecules are important components of biological processes and a number of these biomolecular interactions have been targeted for the development of novel therapeutic drugs. p53 is a transcription factor and tumor suppressor protein that is activated in response to cellu ...

PDF 1 MB

指南

Alpha Protein-Protein Interaction Quick Start Guide

Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored ...

PDF 380 KB
User's Guide To Alpha Assays Protein:Protein Interactions

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners ...

PDF 2 MB
ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

PDF 1 MB

海報

Development of a Homogenous and Label-less AlphaScreen Based Platform to Measure MEK2 Activity

Mitogen-activated protein kinases, MAPKs, are constituents of numerous signal transduction pathways, and are activated by protein kinase cascades. There is great interest in finding compounds that target components of the MAPK pathways as possible treatment for inflammatory diseases and cancer. Here ...

PDF 395 KB
Using AlphaScreen to Detect GST-Protein Substrate Phosphorylation

Protein kinases are directly implicated in many human diseases, including inflammatory diseases and cancer; thus, kinases are promising therapeutic targets. In kinase screening and deorphanization studies, the use of protein substrates constitutes a major advantage over peptide substrates. The Alph ...

PDF 1 MB
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