For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
These beads can be used to capture GST-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:
In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.
| 抗體偶聯物 | Glutathione |
|---|---|
| 自動化兼容 | Yes |
| 珠型或核心珠型 | Alpha Donor |
| 產品品牌名稱 | AlphaLISA |
| 運輸條件 | 藍冰 |
| 產品尺寸 | 25 mg |
Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.
The interactions and bindingof proteins are implicated in a large number of biological processes. The needfor an efficient, highly sensitive assay to study large protein interactions is increasingly important. Alpha Technology is a highly flexible, homogeneous, no-wash assay ideal for the measuremen ...
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Binding events between biomolecules are important components of biological processes and a number of these biomolecular interactions have been targeted for the development of novel therapeutic drugs. p53 is a transcription factor and tumor suppressor protein that is activated in response to cellu ...
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored ...
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners ...
This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.
Mitogen-activated protein kinases, MAPKs, are constituents of numerous signal transduction pathways, and are activated by protein kinase cascades. There is great interest in finding compounds that target components of the MAPK pathways as possible treatment for inflammatory diseases and cancer. Here ...
Protein kinases are directly implicated in many human diseases, including inflammatory diseases and cancer; thus, kinases are promising therapeutic targets. In kinase screening and deorphanization studies, the use of protein substrates constitutes a major advantage over peptide substrates. The Alph ...
