For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Alpha Donor beads coated with nickel chelate. These beads can be used to capture His-tagged proteins and biomolecules, and can be used in conjunction with AlphaScreen, AlphaLISA, or AlphaPlex Acceptor beads to create no-wash assays for:
In a typical Alpha assay, 1 mg of Donor beads is sufficient to run 1,000-2,000 wells using a 25 µL reaction volume. Bead concentration can be adjusted for optimal performance.
| 抗體偶聯物 | Nickel chelate |
|---|---|
| 自動化兼容 | Yes |
| 珠型或核心珠型 | Alpha Donor |
| 檢測方法 | Alpha |
| 實驗類型 | In vitro |
| Format | Microplates |
| 產品品牌名稱 | AlphaLISA |
| 運輸條件 | 藍冰 |
| 產品尺寸 | 1 mg |
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Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored ...
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners ...
This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.
AlphaScreen Nickel Chelate Donor Beads at 5 mg/mL in 25 mM Hepes, pH 7.4, 100 mMNaCl supplemented with 0.05% Proclin-300 as a preservative.
