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3-Amino-3-Deoxydigoxigenin-9-dCTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

貨號 NEL562001EA
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詳細信息

Aminodigoxigenin (DIG) labeled deoxycytidine triphosphate (dCTP) is useful for labeling with a variety of methods including nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. Aminodigoxigenin-labeled products may be detected with anti-DIG antibody conjugates. For the highest sensitivity in detection, we recommend using an anti-DIG-HRP conjugate with tyramide signal amplification (TSA.

The number Ə' in the compound name refers to the length of the linker arm between dCTP and the label.

規格

Buffer 10 mM Tris-HCl, pH 7.6, 1 mM EDTA
濃度 1 mM
檢測方法 Indirect
半抗原 DIG
核苷酸 dCTP
運輸條件 環境
產品尺寸 25 nmol
體積 25 µL
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應用文獻

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TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.

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