Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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Aminodigoxigenin (DIG) labeled labeled dideoxyguanosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Aminodigoxigenin-labeled products may be detected with anti-DIG antibody conjugates. For the highest sensitivity in detection, we recommend using an anti-DIG-HRP conjugate with tyramide signal amplification (TSA).
The number "9" in the compound name refers to the length of the linker arm between dCTP and the label.
Buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
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濃度 | 1 mM |
檢測方法 | Indirect |
半抗原 | DIG |
核苷酸 | ddGTP |
運輸條件 | 環境 |
產品尺寸 | 25 nmol |
體積 | 25 µL |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.