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Anti-6xHis AlphaLISA Acceptor beads, 5 mg

AlphaLISA® Acceptor beads conjugated to anti-His antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine regions at the amino- or carboxy- terminus. These beads can be used to capture His-tagged proteins and peptides for AlphaLISA no-wash assays.

This is a replacement for AL128.

For research use only. Not for use in diagnostic procedures.

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產品尺寸
AL178C
250 μg
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AL178M
5 mg
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AL178R
25 mg
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詳細信息

These beads can be used to capture His-tagged proteins and peptides, and can be used in conjunction with Alpha Donor beads to create AlphaLISA® no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

In a typical AlphaLISA assay, 1 mg of Acceptor beads is sufficient to run 1,000-2,000 wells using a 50 μL reaction volume.

Features:

  • No-wash steps, no separation steps
  • Ease-of-use: few addition steps, fast assay development
  • Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
  • Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
  • High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders

AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym "Alpha&quit; stands for amplified luminescent proximity homogeneous assay. As the name implies, some of the key features of these technologies are that they are non-radioactive, homogeneous proximity assays. Binding of molecules captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent/fluorescent signal. To understand how a signal is produced, one must begin with an understanding of the beads. AlphaScreen and AlphaLISA assays require two bead types: Donor beads and Acceptor beads. Each bead type contains a different proprietary mixture of chemicals, which are key elements of the AlphaScreen technology. Donor beads contain a photosensitizer, phthalocyanine, which converts ambient oxygen to an excited and reactive form of O2, singlet oxygen, upon illumination at 680 nm. Please note that singlet oxygen is not a radical; it is molecular oxygen with a single excited electron. Like other excited molecules, singlet oxygen has a limited lifetime prior to falling back to ground state. Within its 4 μsec half-life, singlet oxygen can diffuse approximately 200 nm in solution. If an Acceptor bead is within that proximity, energy is transferred from the singlet oxygen to thioxene derivatives within the Acceptor bead, subsequently culminating in light production at 520-620 nm (AlphaScreen) or at 615 nm (AlphaLISA). In the absence of an Acceptor bead, singlet oxygen falls to ground state and no signal is produced. This proximity-dependent chemical energy transfer is the basis for AlphaScreen's homogeneous nature.

規格

抗體偶聯物 Anti-6X His
檢測技術 Alpha
自動化兼容 Yes
珠型或核心珠型 AlphaLISA Acceptor
檢測方法 Alpha
實驗類型 In vitro
產品品牌名稱 AlphaLISA
運輸條件 藍冰
產品尺寸 5 mg
資源
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指南

ELISA to AlphaLISA Immunoassay Conversion Guide

This guide presents the simple conversion of an ELISA or other immunoassay to an AlphaLISA® immunoassay.

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User's Guide To Alpha Assays Protein:Protein Interactions

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