BCIP/NBT substrate detects alkaline phosphatase in blotting and slide applications. Available in ready to use solution, it deposits a permanent dark purple stain on membrane sites bearing phosphatase and with the combination of BCIP(5-bromo-4-chloro-3indolyl-phosphate and NBT(nitrobluetetrazoleum) produces much higher sensitivity than either reagent separately.
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Use enough reagent to completely cover the membrane surface or at least 0.25 ml/cm2 of membrane.
This substrate solution is formulated for use on PolyScreen® PVDF Transfer Membrane, nitrocellulose membranes and the nylon membranes GeneScreen™ Hybridization Transfer Membrane and GeneScreen Plus® Hybridization Transfer Membrane.
| Detection Method | Absorbance/Colorimetric |
|---|---|
| Quantity in a Package Amount | 2.0 Units |
| Shipping Condition | Ambient |
| Unit Size | 250 mL |
With applications in biotechnology, molecular biology, proteomics, and more, western blotting is the most widely used technique for detecting protein expression levels in cells.
Our wide range of western blotting solutions gives you the highest level of sensitivity, specificity, and select ...