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Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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Biotin labeled dideoxyguanosine triphosphate (ddUTP) is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Biotin-labeled products may be detected with streptavidin conjugates. For the highest sensitivity in detection, we recommend using a streptavidin-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between ddGTP and the label.
| Buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| 濃度 | 1 mM |
| 檢測方法 | Indirect |
| 半抗原 | Biotin |
| 核苷酸 | ddUTP |
| 運輸條件 | 環境 |
| 產品尺寸 | 25 nmol |
| 體積 | 25 µL |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
