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Biotin-11-ddUTP

Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.

貨號 NEL547001EA
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詳細信息

Biotin labeled dideoxyguanosine triphosphate (ddUTP) is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Biotin-labeled products may be detected with streptavidin conjugates. For the highest sensitivity in detection, we recommend using a streptavidin-HRP conjugate with tyramide signal amplification (TSA).

The number “11” in the compound name refers to the length of the linker arm between ddGTP and the label.

規格

Buffer 10 mM Tris-HCl, pH 7.6, 1 mM EDTA
濃度 1 mM
檢測方法 Indirect
半抗原 Biotin
核苷酸 ddUTP
運輸條件 環境
產品尺寸 25 nmol
體積 25 µL
資源
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