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DELFIA Eu-N1 Rabbit Anti-Mouse-IgG antibody, 50 µg

Europium-labeled anti-mouse IgG antibody, for capturing antibodies in DELFIA® TRF assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
AD0124
50 µg
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AD0207
1 mg
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詳細信息

Generic DELFIA reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA N1-chelate. They are suitable for highly sensitive endpoint measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for quantitative immunoassays.

DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology that provides a unique alternative to traditional ELISA. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA rabbit anti-mouse IgG antibody reagents are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background.

The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to an increased signal-to-noise ratio. Sensitivity of the rabbit anti-mouse IgG antibody assay is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

規格

抗體偶聯物 Anti-mouse IgG
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), DELFIA TRF
實驗類型 In vitro
熒光基團 Eu-N1
Format Microplates
產品品牌名稱 DELFIA
運輸條件 藍冰
產品尺寸 50 µg
資源
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應用簡報

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

PDF 1 MB

操作手冊

Generic DELFIA Reagents

Technical Information, Kit Inserts, AD0038, AD0039, AD0040, AD0041, AD0046, AD0047, AD0048, AD0049, AD0050, AD0053, AD0054, AD0105, AD0106, AD0108, AD0109, AD0112, AD0113, AD0124, AD0159, AD0160, AD0207, AD0250, AD0251

PDF 90 KB
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