DELFIA® Lysis buffer is intended for use in cell-mediated DELFIA cytotoxicity assay (for the determination of the maximum release). The product forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, and is also available as a separate product.
true falseFor research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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DELFIA® time-resolved fluorescence (TRF) cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
| Automation Compatible | Yes |
|---|---|
| Detection Method | Time-Resolved Fluorescence (TRF), DELFIA TRF |
| Experimental Type | In vitro |
| Format | Microplates |
| Product Brand Name | DELFIA |
| Shipping Condition | Blue Ice |
| Unit Size | 30 mL |
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