Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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This dinitrophenol (DNP) labeled analog of uridine triphosphate (UTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. DNP labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. DNP-labeled products may be detected with anti-DNP antibody conjugates. For the highest sensitivity in detection, we recommend using a anti-DNP-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between UTP and the label.
| Buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| 濃度 | 10 mM |
| 檢測方法 | Indirect |
| 半抗原 | DNP |
| 核苷酸 | UTP |
| 運輸條件 | 環境 |
| 產品尺寸 | 250 nmol |
| 體積 | 25 µL |
TSA reduces exposure timein an imaging assay for PKCa. Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility.
