DOPlify^® WGA V2 Kit

The DOPlify^® WGA V2 kit offers the advantage of full mitochondrial DNA coverage and the ability to add custom primers to specially amplify regions of interest. Products of the DOPlify^® WGA V2 kit can be used for a wide range of downstream processes, workflows, and NGS applications. The technology captures exclusive know-how for the lysis and whole genome amplification, with reagents provided in an easy-to-use kit format and less than 3 hour protocol time. Learn More.

• Fast whole genome amplification with protocol time < 3 hours
• Reduced contamination risk with only 2 tube openings required and 3 reagents to add
• Can be fully automated with the JANUS^® G3 liquid handler
• No amplification of NTC
• 1.25 μg of DNA from one cell

CITATIONS:

1. Publications that Cite Using the DOPlify^® Kit for Whole Genome Amplification Deleye, L., Tilleman, L., Plaetsen, A. V., Cornelis, S., Deforce, D., & Nieuwerburgh, F. V. (2017). Performance of four modern whole genome amplification methods for copy number variant detection in single cells. Scientific Reports, 7(1). doi:10.1038/s41598-017-03711-y.
2. Deleye, L., Plaetsen, A. V., Weymaere, J., Deforce, D., & Nieuwerburgh, F. V. (2018). Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells. Scientific Reports,8(1). doi:10.1038/s41598-018-19509-5.
3. Mai, A. D., Harton, G. L., Quang, V. N., Van, H. N., Thi, N. H., Thuy, N. P., . . . Quoc, Q. T. (2020). Development and clinical application of a preimplantation genetic testing for monogenic disease (PGT-M) for beta thalassemia in Vietnam. Journal of Assisted Reproduction and Genetics. doi:10.1007/s10815-020-02006-y.
4. Plaetsen, A. V., Deleye, L., Cornelis, S., Tilleman, L., Nieuwerburgh, F. V., & Deforce, D. (2017). STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods. Scientific Reports, 7(1). doi:10.1038/s41598-017-17525-5.