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LANCE Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 36 (H3K36me2). For use in LANCE® Ultra epigenetic writer and eraser assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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Quantity
TRF0408-D
10 µg
4825.00 USD
 
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TRF0408-M
100 µg
11900.00 USD
 
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Overview

The LANCE® Ultra Europium-anti-di-methyl-Histone H3 Lysine 36 (H3K36me2) antibody was used for the development and optimization of a JMJD2a demethylase assay using a biotinylated Histone H3 (21-44) peptide tri-methylated at lysine 36 as substrate. A technical note describing
the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

Specifications

Antibody Conjugates Anti-H3K36me2
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Format Microplates
Molecular Modification Methylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Therapeutic Area Oncology
Unit Size 100 µg
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Application Brief

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

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