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LANCE Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) Antibody, 100 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 and H4 methylated arginine residues. For use in LANCE® Ultra epigenetic writer and eraser assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
TRF0415-D
10 µg
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TRF0415-M
100 µg
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詳細信息

The LANCE® Ultra Europium-anti-methyl-Histone H3/H4 Arginine (H3/H4Rme pan) antibody was used for the development and optimization of a PRMT4 methylation assay using a biotinylated Histone H3 peptide as substrate. A technical note describing
the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

規格

抗體偶聯物 Anti-methyl arginine
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), TR-FRET
實驗類型 In vitro
熒光基團 Eu-W1024
Format Microplates
分子修飾 Methylation
產品品牌名稱 LANCE Ultra
運輸條件 幹冰
產品尺寸 100 µg
資源
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應用簡報

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