For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The LANCE® Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) antibody was used for the development and optimization of methyltransferase assays.
Formats:
Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.
Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.
| 抗體偶聯物 | Anti-H3K27me3 |
|---|---|
| 自動化兼容 | Yes |
| 檢測方法 | Time-Resolved Fluorescence (TRF), TR-FRET |
| 實驗類型 | In vitro |
| 熒光基團 | Eu-W1024 |
| Format | Microplates |
| 分子修飾 | Methylation |
| 產品品牌名稱 | LANCE Ultra |
| 運輸條件 | 藍冰 |
| 治療領域 | Oncology |
| 產品尺寸 | 10 µg |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
In this technical note, we present the optimization of an epigenetic enzymatic assay using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody(Eu-Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecule ...
