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DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and antibody-dependent cell cytotoxicity (ADCC). This kit has the same assay principle as a chromium release assay, with target cells loaded with a BATDA ligand instead of 51Cr. There is no requirement for specially-engineered cells.
true falseFor research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
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DELFIA time-resolved fluorescence cell cytotoxicity assays offer a non-radioactive method to Chromium 51, for use in cell-mediated cytotoxicity and antibody-dependent cell cytotoxicity (ADCC) studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
Reagents for the assay are also available separately:
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Automation Compatible | Yes |
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Detection Method | Time-Resolved Fluorescence (TRF), DELFIA TRF |
Experimental Type | In vitro |
Format | Microplates |
Product Brand Name | DELFIA |
Shipping Condition | Blue Ice |
Unit Size | 1 Kit |
Discover 爱游戏平台注册登录 's simplified, gentle cell-labelling method for non-radioactive cytotoxicity assays
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Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...
This product is intended for loading of cells to be used in short term cytotoxicity tests performed utilizing time-resolved fluorometry in the detection.