Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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This fluorescein labeled analog of adenosine triphosphate (ATP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Fluorescein labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
The number “12” in the compound name refers to the length of the linker arm between ATP and the label.
| Buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| Concentration | 1 mM |
| Detection Method | Direct |
| Fluorophore | Fluorescein |
| Nucleotide | dATP |
| Shipping Condition | Ambient |
| Unit Size | 25 nmol |
| Volume | 25 µL |
