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Fluorescein-12-ddATP

Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.

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NEL402001EA
605.00 USD
 
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Overview

Fluorescein labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.

Specifications

Buffer 10 mM Tris-HCl, pH 7.6, 1 mM EDTA
Concentration 1 mM
Detection Method Direct
Fluorophore Fluorescein
Nucleotide ddATP
Shipping Condition Ambient
Unit Size 25 nmol
Volume 25 µL
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