Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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Fluorescein labeled dideoxyadenosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations via single base extension.
| Buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| Concentration | 1 mM |
| Detection Method | Direct |
| Fluorophore | Fluorescein |
| Nucleotide | ddATP |
| Shipping Condition | Ambient |
| Unit Size | 25 nmol |
| Volume | 25 µL |
