Fluorescently-labeled nucleotide analogs for enzymatic incorporation, for direct fluorescence detection.
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This fluorescein labeled analog of uridine triphosphate (UTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. Fluorescein labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. Labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
The number ""12"" in the compound name refers to the length of the linker arm between UTP and the label.
| Buffer | 10 mM Tris-HCl, pH 7.6, 1 mM EDTA |
|---|---|
| 濃度 | 1 mM |
| 檢測方法 | Direct |
| 熒光基團 | Fluorescein |
| 核苷酸 | UTP |
| 運輸條件 | 環境 |
| 產品尺寸 | 25 nmol |
| 體積 | 25 µL |
