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Free Fatty Acid FFA1 (GPR40), Human Recombinant, CHO-K1 AequoZen Frozen cells

Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.

This product contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

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ES-652-AF
1297.00 USD
 
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Overview

Frozen Cells validated for calcium testing: Free Fatty Acid, FFA1 (GPR40) subtype, human recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.

Features:

  • Pre-validated batches of ready-to-use cells for calcium testing
  • Optimized to alleviate biological variability
  • Step-by-Step protocols for culture-free functional testing on our validated cells
  • Affordable access to small aliquots of cells (minimum size: 10 million cells/vial)
  • Ready-to-use cells for Profiling, Pharmacology or Screening applications
  • Up-scaling and customized developments

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.

Specifications

Assay Target Class GPCR
Assay Target Type Cell line
Assay Validated Calcium Luminescence
G-Alpha Natural Receptor Gq/G11
Host CHO-K1
Product Brand Name AequoZen
Receptor Type Free Fatty Acid
Second Messenger Release Calcium flux
Shipping Condition Dry Ice
Therapeutic Area Metabolic, Cancer
Unit Size 10 million cells
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Application Note

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which ...

PDF 607 KB
Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels

Aequorin-based Ca2+ assays represent a new paradigm in drug discovery research for cell-based assays for Ca2+-coupled GPCRs and ion channels. In the aequorin assay, cells co-expressing apoaequorin and the target receptor are first incubated with the co-factor coelenterazinein order to reconstitute t ...

PDF 152 KB

Brochure

Radioligand Selection Guide

Discover a comprehensive collection of GPCR cell lines, membrane preparations, and complementary radioligands designed to drive your GPCR research. Utilize this selection guide to easily identify:

  • Cell lines available for specific receptors
  • Assays that cell lines are validat ...

PDF 1 MB

Flyer

Aequorin FLIPR

Aequorin and Photina cell lines – the alternative calcium flux assay. PDL-coated microplates improve your performance

PDF 1 MB
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