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GrowDase Cellulase enzyme 2.5 ml

GrowDase enzyme breaks down GrowDex® hydrogel to form a solution in an easy one- step efficient cell recovery process. 3D cell structures such as spheroids, organoid or biopsies are retained with no impact on cell viability or functionality. Liberated cells can be used in gene or protein expression studies or for future experiments.

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Unit Size
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900102001
1.25mL
136.00 USD
 
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900102002
2.5mL
179.00 USD
 
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Overview

GrowDase is a purified mixture of cellulase enzymes that has been specifically developed to break down the nano-cellulose fibrils present in GrowDex into soluble glucose. The process is simple, enzyme is mixed with the sample and incubated at 37°C until the GrowDex has been fully reduced to glucose. Enzymatic removal allows cells to be recovered from the matrix efficiently, retain their 3D structure, e.g., spheroid or organoid, intact and without adverse affect on cell functionality. The amount of GrowDase required for cell recovery is dependent on the amount of GrowDex (cellulose) present in the sample. The product is supplied sterilized.

Storage and shelf-life:

  • Storage: 4-22 °C before opening, 4-8 °C after opening.
  • Shelf life: 6 months
  • Shelf life after opening: 3 months

Concentration

  • GrowDase is supplied at a concentration of 10mg/mL.

Specifications

Shipping Condition Ambient
Unit Size 2.5mL
Resources, Events & More
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Data Sheet

GrowDase Instructions for Use

This document provides instructions for use, safety information, storage instructions, ordering and contact information for GrowDase.

PDF 99 KB

Guide

GrowDex Technical Protocols

This guide describes a number of experimental protocols performed using GrowDex hydrogels.

PDF 928 KB
Guide to Reducing the Cost and Time to Set-up Reproducible HTS/HCS with 3D Cell Models

Download our guide to reducing the costs and time required to set up reproducible high-throughput and high-content screens using 3D cell culture models.

Topics include:

  • Opportunities and challenges in 3D cell culture models for HTS and HCS
  • Challenges of scaling up 3D HTS/ ...

PDF 616 KB
How To Transfer From Animal-Derived Matrices to GrowDex - 3D Cell Culture Matrix

Animal-derived matrices for 3D cell culture are often poorly defined and suffer from lot-to-lot variability. This variability ultimately affects the reproducibility of the experiment, which is an essential element for reliable research studies.

Natural, reliable and ready-to-use, GrowDex

PDF 2 MB

Scientific Papers

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