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AequoScreen Cells, opioid kappa receptor, human recombinant, HEK293

Stable, recombinant AequoScreen® cell line expressing aequorin and the Opioid kappa receptor, human recombinant in HEK293 host cells.

貨號 6110558-K
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Cell Line Terms and Conditions must be accepted before orders are placed. Additional agreement needed for commercial use.

詳細信息

爱游戏平台注册登录 ’s AequoScreen® double transfected cell lines are provided in two vials of frozen cells, each containing ∽2.5 x 106 cells. Detailed product information as well as recommended cell culture conditions and validation assay date are available for each cell line.

The AequoScreen technology is a generic GPCR technology that can be used with Gs, Gi, and Gq-coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z′, CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

爱游戏平台注册登录 ’s AequoScreen cell lines require accepted cell line Terms and Conditions prior to order processing. Additional agreement needed for commercial use. Contact us using the Request More Information button above for additional information regarding required documentation.

規格

檢測目標類 GPCR
檢測驗證 Calcium Luminescence
自動化兼容 Yes
宿主細胞 HEK293
產品品牌名稱 AequoScreen
受體類型 Opioid
運輸條件 幹冰
特殊訂購信息 Requires accepted Terms and Conditions. Additional agreement needed for commercial use.
產品尺寸 5 million cells
資源
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產品手冊

Take the Stress Out of Functional Testing - AequoZen and cAMPZen ready-to-use cells

爱游戏平台注册登录 ’s validated, ready-to-use AequoZen™or cAMPZen™ frozen, irradiated cells make it easier for you to perform functional testing of GPCRs. Just thaw and use! Reliable, convenient AequoZen cells for aequorin calcium testing or cAMPZen cells for cAMP testing let you get the testing done. So yo ...

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應用文獻

AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines

Aequorin is a photo protein originating from the jellyfish Aequorea Victoria. The apo-enzyme (apoaequorin) is a 21 kD protein, which requires a hydrophobic prosthetic group, coelenterazine, to be converted to aequorin,the active form of the enzyme. This enzyme possesses 3 calcium binding sites which ...

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