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LANCE Eu-W1024 Anti-Human IgG, 10µg

Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

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AD0212
10 µg
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AD0074
50 µg
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AD0075
1 mg
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詳細信息

Eu-W1024 labeled anti-human IgG antibodies for LANCE® assays where analyte concentration is < 1-10 nmol/L and assay pH > 7. This affinity purified mouse monoclonal IgG2b antibody is specific for the Fc-part of human IgG. The antibodies recognize all IgG subclasses and cross-react with rabbit immunoglobulins. It is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

規格

抗體偶聯物 Anti-human IgG
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), TR-FRET
實驗類型 In vitro
熒光基團 Eu-W1024
Format Microplates
產品品牌名稱 LANCE
運輸條件 藍冰
產品尺寸 10 µg
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應用簡報

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操作手冊

LANCE generic reagents

Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.

PDF 101 KB
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