For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Eu-W1024 labeled anti-human IgG antibodies for LANCE® assays where analyte concentration is < 1-10 nmol/L and assay pH > 7. This affinity purified mouse monoclonal IgG2b antibody is specific for the Fc-part of human IgG. The antibodies recognize all IgG subclasses and cross-react with rabbit immunoglobulins. It is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution.
Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
抗體偶聯物 | Anti-human IgG |
---|---|
自動化兼容 | Yes |
檢測方法 | Time-Resolved Fluorescence (TRF), TR-FRET |
實驗類型 | In vitro |
熒光基團 | Eu-W1024 |
Format | Microplates |
產品品牌名稱 | LANCE |
運輸條件 | 藍冰 |
產品尺寸 | 10 µg |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
Generic LANCE reagents are intended for setting up TR-FRET based assays using Eu-chelate label as a donor and APC-labeled reagent as an acceptor.