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LANCE Ultra cAMP Detection Kit, 50,000 assay points | 爱游戏平台注册登录
爱游戏平台注册登录
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LANCE Ultra cAMP Detection Kit, 50,000 assay points

The LANCE® Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
TRF0262
1,000 assay points
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TRF0263
10,000 assay points
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TRF0264
50,000 assay points
更多
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詳細信息

爱游戏平台注册登录 's LANCE® Ultra cAMP assay offers unmatched sensitivity and S/B using a simple TR-FRET protocol. Ideal for any Gs- or Gi-coupled GPCR assay, this Ultra-sensitive cAMP assay is supreme when working with low cell numbers, endogenous receptors, or difficult Gi-coupled receptors.

Features:

  • Unmatched assay sensitivity and signal stability-even with overnight incubation
  • Optimized screening of difficult targets and Gαi antagonists
  • Easier detection of cAMP response of endogenous receptors-use fewer cells per well
  • Robust sensitivity when miniaturized to 1536-well format for uHTS
  • Trusted results: stable pharmacology over time with consistent rank order potencies
  • Reproducible results with highest Z' values

The LANCE Ultra cAMP assay is a homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by G-protein coupled receptors (GPCRs).

The assay is based on the competition between the europium (Eu) chelate-labeled cAMP tracer and cellular cAMP for binding sites on cAMP-specific monoclonal antibodies labeled with the ULight dye. When antibodies are bound to the Eu-labeled cAMP tracer, light pulse at 320 or 340 nm excites the Eu chelate molecule of the tracer. The energy emitted by the excited Eu chelate is transferred by FRET to ULight molecules on the antibodies, which in turn emit light at 665 nm.

規格

檢測目標 cAMP
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), TR-FRET
實驗類型 In vitro
Format Microplates
產品品牌名稱 LANCE Ultra
第二信使釋放 cAMP
運輸條件 藍冰
治療領域 Metabolic
產品尺寸 50,000 assay points
資源
  • 所有

產品手冊

應用文獻

Phosphodiesterase 4 assay using the new LANCE® Ultra cAMP kit

Cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of 3',5'-cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) to their corresponding 5’-nucleotide monophosphates, AMP and GMP.

PDF 1 MB

指南

LANCE® Ultra cAMP Assay Development Guide

The LANCE® Ultra cAMP kit is intended for the quantitative determination of 3’,5’-cyclic adenosine monophosphate (cAMP) in cell lysate and cellular membrane samples.

PDF 970 KB

操作手冊

白皮書

Cell-Based Assays: Purposeful Screens for Better Results

Over these last few decades there has been a growing trend in drug discovery to use cellular systems and functional assays, in addition to biochemical assays, for the characterization of new potential therapeutics. The ability to study the interaction between a candidate drug and its target withi ...

PDF 1 MB
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