IFN-α (human) LANCE Ultra TR-FRET Detection Kit, 500 assay points

The LANCE Ultra Human IFN-α Detection Kit is designed for detection and quantitation of human interferon α in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample).

• No-wash steps, no separation steps
• TR-FRET technology
• Sensitive detection
• High reproducibility
• Faster time-to-results
• Easy automation
• 96-well, 384-well, and 1536-well formats

LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight^™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

Interferon-alpha (IFN-α) is a cytokine involved in innate immune response against viral infection. It is produced by leukocytes and other cell types like plasmacytoid dendritic cells. There are many IFN-α subtypes, with molecular weights ranging from 15 to 21 kDa. They all possess the same antiviral activity, but may differ in their relative biological activities. IFN-α also exhibits several antiproliferative and antitumor activities, making it one of the most used cytokine treatments in cancer patients suffering from hairy cell leukemia, renal carcinoma, Kaposi’s sarcoma, and other malignancies. Under physiological conditions, a low level of IFN-α is detected. However, its production is markedly enhanced during infections and various pathological situations, making IFN-α a major disease marker.