爱游戏平台注册登录
check quantity

Phospho-AMPKα (Thr172) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

LANCE Ultra phospho-AMPKα (Thr172) kits are designed for the detection of phosphorylated AMPKα in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
TRF4027C
500 Assay Points
更多
TRF4027M
10,000 Assay Points
更多
Request Technical Support

請輸入有效數量

請登錄以添加收藏夾。

購物車為空

詳細信息

Please note control lysates are sold separately, catalog number TRF4027S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

AMPKα is an isoform of AMP-activated protein kinase which plays a role in cellular energy homeostasis. Once bound by AMP or ADP, AMPK activations results in the stimulation of hepatic fatty acid oxidation, ketogenesis, and glucose uptake amongst other metabolic functions. Once the alpha subunit is phosphorylated at the T172 position, AMPK is considered activated.

規格

檢測目標 AMPKα
檢測目標類 Phosphoprotein
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), TR-FRET
產品品牌名稱 LANCE Ultra
運輸條件 藍冰
治療領域 Neuroscience, Cardiovascular
產品尺寸 10,000 Assay Points
資源
  • 所有

產品手冊

應用簡報

Eight Limitations of ELISA and How to Overcome Them Using Alternative Technologies

The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...

PDF 1 MB
Baidu
map