For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Please note control lysates are sold separately, catalog number TRF4027S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
AMPKα is an isoform of AMP-activated protein kinase which plays a role in cellular energy homeostasis. Once bound by AMP or ADP, AMPK activations results in the stimulation of hepatic fatty acid oxidation, ketogenesis, and glucose uptake amongst other metabolic functions. Once the alpha subunit is phosphorylated at the T172 position, AMPK is considered activated.
檢測目標 | AMPKα |
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檢測目標類 | Phosphoprotein |
自動化兼容 | Yes |
檢測方法 | Time-Resolved Fluorescence (TRF), TR-FRET |
產品品牌名稱 | LANCE Ultra |
運輸條件 | 藍冰 |
治療領域 | Neuroscience, Cardiovascular |
產品尺寸 | 500 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...