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Please note control lysates are sold separately, catalog number TRF4026S.
Formats:
LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).
LCK is a cytoplasmic phosphoprotein from the Src family of non-receptor tyrosine kinases. LCK regulates T cell activation by associating with CD4 and CD8 surface receptors on T cells. Phosphorylation at Y394 enhances kinase activity. LCK is associated with Immunodeficiency 22 and CD45 deficiency. No-death receptor apoptosis is also involves LCK.
| Assay Target | LCK |
|---|---|
| Assay Target Class | Phosphoprotein |
| Automation Compatible | Yes |
| Detection Method | Time-Resolved Fluorescence (TRF), TR-FRET |
| Product Brand Name | LANCE Ultra |
| Shipping Condition | Blue Ice |
| Unit Size | 500 Assay Points |
The introduction of enzyme-linked immunosorbent assays (ELISAs) in the early 1970’s offered researchers a non-radiometric immunoassay platform without compromising sensitivity. Over the last 50 years scientists have made huge strides in disease research and drug discovery and a demand for greater ...
