Phospho-TBK1 (Ser172) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

Please note control lysates are sold separately, catalog number TRF4031S.

Formats:

• Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
• Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE^® and LANCE^® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

TANK-binding kinase 1 (TBK1) is auto-phosphorylated at Ser172. It contributes to multiple pathways. Activation of TBK1 leads to the activation of the TLR3/4 and cGAS-STNG signaling pathways. Ultimately activation of TBK1 leads to IRF3 activation. Phosphorylation of optineurin and p62 by TBK1 marks proteins to be directed to the autophagosome.