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Phospho-ZAP-70 (Tyr319) LANCE Ultra TR-FRET Detection Kit, 500 assay points

LANCE Ultra phospho-ZAP-70 (Tyr319) kits are designed for the detection of phosphorylated ZAP-70 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
TRF4024C
500 Assay Points
更多
TRF4024M
10,000 Assay Points
更多
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詳細信息

Please note control lysates are sold separately, catalog number TRF4024S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

ZAP-70 (Zeta-chain-associated protein kinase 70) is normally expressed near the surface membrane of T-cells and natural killer cells, but levels in B-cells can be an indicator for various forms of leukemia. ZAP-70 is recruited to the TCR/CD3 complex in order to be phosphorylated. Once phosphorylated, ZAP-70 promotes downstream phosphorylation of proteins such as SLP76 and ERK.

規格

檢測目標 Zap70
檢測目標類 Phosphoprotein
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), TR-FRET
產品品牌名稱 LANCE Ultra
運輸條件 藍冰
治療領域 Autoimmunity, Oncology, Immuno-oncology
產品尺寸 500 Assay Points
資源
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應用簡報

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