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Phospho-SMAD3 (Ser423/425) LANCE Ultra TR-FRET Cellular Detection Kit, 10,000 Assay Points

LANCE Ultra phospho-SMAD3 (Ser423/425) kits are designed for the detection of SMAD3 (phosphorylated at residue S423/S425) in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
TRF4014C
500 Assay Points
1947.00 USD
 
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TRF4014M
10,000 Assay Points
8800.00 USD
 
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Overview

Please note control lysates are NOT included in kits. Control lysates are sold separately, catalog number TRF4014S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

Specifications

Assay Pathway TGFß/SMAD
Assay Target SMAD3
Assay Target Class Phosphoprotein
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Therapeutic Area Metabolic
Unit Size 10,000 Assay Points
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