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Phospho-STAT3 (Tyr705) LANCE Ultra TR-FRET Cellular Detection Kit, 500 Assay Points

LANCE Ultra phospho-STAT3 (Tyr705) kits are designed for the detection of phosphorylated STAT3 in cell lysates using a simple, homogeneous LANCE Ultra assay (no wash steps). This assay is compatible with both adherent and suspension cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
TRF4004C
500 Assay Points
更多
TRF4004M
10,000 Assay Points
更多
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詳細信息

Please note control lysates are NOT included in the kits. Control lysates are sold separately, catalog number TRF4004S.

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume.
  • Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000).

STAT3 is an important transcriptional activator, and is involved in regulating cytokines and growth factor receptors. STAT3 is phosphorylated at Tyr-705 by the JAK pathway, upon which STAT3 will dimerize, translocate to the nucleus, and bind DNA. In some cancers STAT3 is shown to be constitutively activated. It has been implicated in both anti-apoptotic and oncogenic pathways.

規格

分析途徑 JAK/STAT
檢測目標 STAT3
檢測目標類 Phosphoprotein
自動化兼容 Yes
檢測方法 Time-Resolved Fluorescence (TRF), TR-FRET
產品品牌名稱 LANCE Ultra
運輸條件 藍冰
治療領域 Autoimmunity, Neuroscience, Respiratory
產品尺寸 500 Assay Points
資源
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產品手冊

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