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AlphaScreen® Rabbit IgG Detection Kit, 50,000 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-rabbit IgG conjugated AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
Your Price
Quantity
6760607C
500 assay points
269.00 USD
 
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6760607M
10,000 assay points
4316.00 USD
 
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6760607R
50,000 assay points
20700.00 USD
 
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Overview

This bead kit can be used for many applications, including:

  • Antibody detection assays
  • Protein detection assays
  • Protein-protein interaction assays
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is an anti-species antibody, and associates with the corresponding AlphaScreen anti-species Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FITC probe provided in the kit.

Specifications

Antibody Conjugates Streptavidin/Anti-rabbit IgG
Automation Compatible Yes
Product Brand Name AlphaScreen
Shipping Condition Blue Ice
Unit Size 50,000 assay points
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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
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