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Alpha SureFire Ultra Multiplex Phospho (Thr821/826)/Total Rb Assay Kit, 50,00 Assay Points

Alpha Terbium SureFire Ultra Multiplex MPSU

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The AlphaLISA^® SureFire^® Ultra^™ Multiplex phospho-Rb (Thr821/826) + Total Rb assay kit is used to measure both the phosphorylation of Rb and total levels of endogenous Rb in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Rb phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

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For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

MPSU-PTRB-N500

500 Assay Points

3827.00 USD

MPSU-PTRB-N10K

10,000 Assay Points

23200.00 USD

MPSU-PTRB-N50K

50,000 Assay Points

72600.00 USD

MPSU-PTRB-N-HV

100 Assay Points

1132.00 USD

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Overview
Resources, Events & More
SDS, COAs & More

Overview

The Alpha SureFire^® Ultra^™ Multiplex p-Rb (Thr821/826) (Eu) + Total Rb ssay kit is used to measure both phosphorylated and total levels of endogenous Rb in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Rb analysis, and the 545 nm (Tb) signal corresponds to the total Rb analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

No-wash steps, no separation steps
Multiplexing assay
Sensitive detection
No interference from exogenous antibodies
Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure^™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2^™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Points (200-50,000)	50000 Assay Points
Assay Target	RB
Assay Target Class	Phosphoprotein, Protein
Assay Technology	Alpha
Automation Compatible	Yes
Detection Method	Alpha
Experimental Type	In vitro
Product Brand Name	AlphaLISA SureFire Ultra Multiplex
Shipping Condition	Blue Ice
Therapeutic Area	Oncology
Unit Size	50,000 Assay Points

Resources, Events & More

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Brochure

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