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Alpha SureFire Ultra Multiplex Phospho (Thr821/826)/Total Rb Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex phospho-Rb (Thr821/826) + Total Rb assay kit is used to measure both the phosphorylation of Rb and total levels of endogenous Rb in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Rb phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Part Number
Unit Size
List Price
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MPSU-PTRB-N500
500 Assay Points
3827.00 USD
 
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MPSU-PTRB-N10K
10,000 Assay Points
23200.00 USD
 
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MPSU-PTRB-N50K
50,000 Assay Points
72600.00 USD
 
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MPSU-PTRB-N-HV
100 Assay Points
1132.00 USD
 
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Overview

The Alpha SureFire® Ultra Multiplex p-Rb (Thr821/826) (Eu) + Total Rb ssay kit is used to measure both phosphorylated and total levels of endogenous Rb in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated Rb analysis, and the 545 nm (Tb) signal corresponds to the total Rb analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

Specifications

Assay Points (200-50,000) 100 Assay Points
Assay Target RB
Assay Target Class Phosphoprotein, Protein
Assay Technology Alpha
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA SureFire Ultra Multiplex
Shipping Condition Blue Ice
Therapeutic Area Oncology
Unit Size 100 Assay Points
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Application Brief

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Brochure

Alpha Technology Solutions

Product brochure for the Alpha Technology, a versatile, no wash, homogeneous assay technology that's suitable for a broad range of applications.

PDF 2 MB
Drug Discovery Screening Solutions Brochure

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

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