The AlphaLISA® SureFire® Ultra™ Multiplex phospho-ribosomal protein S6 (Ser240/244) + Total ribosomal protein S6 assay kit is used to measure both the phosphorylation of ribosomal protein S6 and total levels of endogenous ribosomal protein S6 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure S6 ribosomal protein phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.
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The Alpha SureFire® Ultra™ Multiplex p-ribosomal protein S6 (Ser240/244) (Eu) + Total ribosomal protein S6 (Tb)assay kit is used to measure both phosphorylated and total levels of endogenous ribosomal proteinS6 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated ribosomal protein S6 analysis, and the 545 nm (Tb) signal corresponds to the total ribosomal protein S6 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.
In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.
| Assay Points (200-50,000) | 10000 Assay Points |
|---|---|
| Assay Target | Ribosomal Protein S6 |
| Assay Target Class | Phosphoprotein, Protein |
| Assay Technology | Alpha |
| Automation Compatible | Yes |
| Detection Method | Alpha |
| Experimental Type | In vitro |
| Product Brand Name | AlphaLISA SureFire Ultra Multiplex |
| Shipping Condition | Blue Ice |
| Therapeutic Area | Oncology, Inflammation, Metabolic |
| Unit Size | 10,000 Assay Points |
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