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Alpha SureFire Ultra Multiplex Phospho (Ser727)/Total STAT1 Assay Kit, 100 Assay Points

The AlphaLISA® SureFire® Ultra Multiplex phospho-STAT1 (Ser727) + Total STAT1 assay kit is used to measure both the phosphorylation of STAT1 and total levels of endogenous STAT1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure STAT1 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells.

For research use only; not for diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

貨號
產品尺寸
MPSU-PTST1-M500
500 Assay Points
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MPSU-PTST1-M10K
10,000 Assay Points
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MPSU-PTST1-M50K
50,000 Assay Points
更多
MPSU-PTST1-M-HV
100 Assay Points
更多
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詳細信息

The Alpha SureFire® Ultra Multiplex p-STAT1 (Ser727) (Eu) + Total STAT1 assay kit is used to measure both phosphorylated and total levels of endogenous STAT1 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated STAT1 analysis, and the 545 nm (Tb) signal corresponds to the total STAT1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

  • No-wash steps, no separation steps
  • Multiplexing assay
  • Sensitive detection
  • No interference from exogenous antibodies
  • Results in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2 agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.

規格

檢測點(200-50,000) 100 Assay Points
檢測目標 STAT1
檢測目標類 Phosphoprotein, Protein
檢測技術 Alpha
自動化兼容 Yes
檢測方法 Alpha
實驗類型 In vitro
產品品牌名稱 AlphaLISA SureFire Ultra Multiplex
運輸條件 藍冰
治療領域 Oncology, Inflammation
產品尺寸 100 Assay Points
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