Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This appl ...

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta® high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated singl ...

Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.

However, implementation of cell painting is not without its challenges - f ...

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As a result, valuable information from precious c ...

Dysregulation of GPCR signaling, particularly calcium signaling, has been implicated in different diseases, e.g. cancer and Alzheimer’s disease. Currently about 34% of all FDA approved drugs target GPCRs indicating their importance for drug discovery, and in vitro cellular GPCR assays are importa ...

Analyzing transport of biliary metabolites is essential to predict pharmacokinetics and hepatotoxicity during drug development. A functional impairment of hepatobilary transporters, such as bile salt export pump (BSEP) and multidrug resistance-associated protein 2 (MRP-2), is strongly associated wit ...

Drug-induced cardiotoxicity is a frequent cause of drug attrition during drug development and can lead to the withdrawal of drugs from the market, so non-invasive approaches that allow quick and reliable assessment of the cardiotoxic potential of a compound are key to improve drug safety.

I ...

Fundamental processes in living cells, such as apoptosis and signal transduction are controlled by proteins, often acting in concert with other protein partners through protein-protein interactions (PPIs). Inappropriate protein-protein recognition can fundamentally contribute to many diseases, inclu ...

Combining CRISPR gene editing and cell painting provides researchers with the ability to correlate genotypes with phenotypes and holds the promise to help understand gene functions in more detail and at greater scale.

This application note demonstrates a harmonized workflow for morphologica ...

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assa ...

Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the ...

The incidence and prevalence of non-alcoholic fatty liver disease (NAFLD), which is characterized by excessive accumulation of lipids within the liver, is high and progressively rising due to increasing obesity and metabolic syndrome prevalence. For some patients, NAFLD can progress to non-alcoho ...

With powerful potential for phenotypic drug discovery, cell painting is being rapidly adopted by leading scientists across the globe. However, implementing cell painting assays can present some challenges.

To help you avoid some of the common pitfalls, we’ve collated answers from our expert ...

Within diabetes research, an evolving area of interest is the identification and development of an efficacious treatment for ß cell loss. The following literature review discusses the use of a Disque platform for evaluating the functionality of multiple drug candidates.

Live-cell imaging, the study of living cells using microscopy, has become a requisite technology in many fields of biomedical research, such as cell biology, developmental biology and cancer research. Also, in drug discovery, researchers adopt live-cell imaging as they look for a more detailed un ...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus that causes COVID-19, the respiratory illness responsible for the COVID-19 pandemic. Studies have shown that patients infected with SARS-CoV-2 with cardiovascular comorbidities are more susceptible to severe infection and t ...

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

CRISPR is transforming research by enabling the modulation of hundreds or thousands of genes in a single experiment to identify genetic pathways, cellular processes, novel therapeutic targets, and to genetically profile existing or potential therapeutics.. This solution guide will help you confid ...

When Non-alcoholic fatty liver disease (NAFLD) progresses to nonalcoholic steatohepatitis (NASH), with inflammation and damage to liver cells it can cause fibrosis, cirrhosis and liver failure or cancer. Despite the prevalence of NAFLD and NASH rising, their remains no approved treatments. But st ...

Find out about our range of integrated solutions for drug discovery screening in this e-brochure.

Our screening solutions for high-throughput screening, phenotypic screening and data analysis help to streamline drug discovery workflows in labs across the globe. Our portfolio includes automat ...

Every day, researchers are furthering our understanding of cancer biology, uncovering new mechanisms driving the disease. These discoveries provide new targeting options that ultimately drive the next generation of therapeutics. With this guide, discover a diverse range of solutions designed to ...

The Opera Phenix® Plus High-Content Screening System is the premier confocal solution for today's most demanding high content applications.

Truly understanding infectious diseases is the first step toward containment and eradication. From biology to breakthrough, our diverse infectious disease product portfolio helps researchers make critical discoveries through genomic, protein, cell, and in vivo analysis.

The need for better profiling is clear, and technological advances are making it possible to optimize clinical trials and ultimately develop precise therapies. Read our precision medicine research ebook and get the clarity you need in your research to identify the right target – for the right ind ...

This case study illustrates how 爱游戏平台注册登录 ’s high-content imaging solutions are enabling therapeutic antibody development. Two steps in the workflow - the clonal B cell selection and the functional antibody characterization - rely on high-content screening using the Operetta^® system. ...

This case study shows how a previously-described neuroprotection assay was easily and directly transferred to the Opera Phenix^® high-content screening system, with a 4-fold decrease in acquisition time. In the assay, primary rat neurons are co-cultured on top of rat-derived astrocytes. ...

Antimicrobial resistance (AMR) is one of today’s major global public health challenges. Recently, there has been renewed investment in the discovery of novel antimicrobials to urgently address the growing number of drug-resistant infections. This case study describes how Professor Gordon Dougan a ...

Download this booklet from The Scientist and 爱游戏平台注册登录 to learn about how the third dimension affects cell behavior, the similarities and differences between 2-D and 3-D culture, common 3-D culture models, and how to image and analyze 3-D culture models.

While 3D cell culture provides unprecedented opportunities for both increased physiological relevance and analysis using a high-content approach, it is also more complex than traditional 2D cell culture. This booklet, from Biocompare and 爱游戏平台注册登录 , will unravel some of the complexities often encou ...

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches ...

Live-cell imaging has evolved from allowing observation of large-scale changes to capturing subtle changes in dynamic cellular processes. Today, live-cell imaging coupled with high-content analysis enables researchers to extract quantitative data in real-time, facilitating new insights in basic l ...

Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell sampl ...

This guide provides instructions on how to find help and manuals for your HCS Hardware/Software (Opera Phenix Plus, Operetta CLS, Harmony).

Advances in high-content cellular imaging (HCI) and analysis have enabled researchers to obtain rich, quantitative data from cells and identify biologically relevant similarities and differences in their morphological profiles. Morphological profiling has been employed in cancer and toxicology re ...

Glioblastoma is the most common type of malignant brain tumor in adults. It is usually very aggressive, with less than 10% of patients expected to survive five years following diagnosis. Traditional treatment strategies are rarely curative, emphasizing the urgent need to discover more effective t ...

Publications in which 爱游戏平台注册登录 high-content analysis and screening systems are used number more than 1000 between 2010 and 2019. Here we feature some of the more recent publications, focussing on Operetta CLS and Opera Phenix, and categorized by application or research area.

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS^™

Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.

In this technical note we describe how to ...

In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. However, one of the greatest challenges in higher throughput 3D imaging is the acquisition of images of solid spheroids, owing to the redu ...

Live cell imaging has gained importance within drug discovery over recent times, as researchers look for more meaningful insights into cellular behavior and function. However, setting up live cell experiments can be challenging, as temperature, CO₂ and evaporation need to be controlled ...

Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS™ and Opera Phenix™ high-content systems, avoid phototoxicity with gentle digital phase cont ...

Automated image acquisition and analysis of tissue sections can be challenging owing to the inherent height variations throughout each specimen, and this can hinder their use in screening applications.

In this technical note we show how PreciScan^™ intelligent image acquisit ...

The Opera Phenix™ high-content screening system’s state of the art hardware, combined with Harmony® imaging and analysis software, improves acquisition and analysis speed, enabling users to perform highly multi-parametric phenotypic screens effectively. In this technical note, learn how the Opera P ...

Simultaneous multi-color imaging is a technology commonly used to increase the speed of high content screening systems. However, one of the main problems arising from this approach is spectral crosstalk which can limit assay sensitivity. To overcome this challenge, we have developed an innovative ...

Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes. However, for 3D imaging, confocal image quality can be limited by a phenomenon known as ‘pinhole crosstalk’ which can lead to blurred images in conventional sp ...

The key parameters in high-content imaging - speed, sensitivity (or intensity) and resolution - cannot be optimized independently as altering one of them influences the others. For example, increasing the resolution by choosing a higher magnification requires imaging of more fields to maintain th ...

There is a huge unmet need for the development of treatments that target the underlying causes of neurodegenerative diseases. To address the high failure rates and low investment returns of traditional neuroscience R&D approaches, companies are increasingly adopting alternative R&D business model ...

There has been a lot of buzz around artificial intelligence, machine learning and deep learning. Is the reality living up to the hype?

In the world of cellular imaging and its application to drug discovery, there is evidence of real progress against some of the critical challenges facing s ...

Phenotypic Drug Discovery Re-Imagined

Cell Painting is a phenotypic screening method and a powerful application of high-content screening technology which combines cell and computational biology to elucidate the behavior of cells under the influence of perturbagens, such as chemical compoun ...

Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. This whitepaper documents a streamlined procedure for getting the most information, as quickly as possible, using solutions from 爱游戏平台注册登录 .

A team of researchers exploits high-content imaging to phenotype the effects of antimicrobial exposure on individual bacteria cells and screen for novel alternatives to existing antimicrobials. Projects include the development of polyclonal antibody serum, monoclonal antibodies, and functional as ...

Researchers are striving to determine where and when relevant bacteria play a role in cancer progression and whether those interactions are localized to the gastrointestinal tract or occur in other tissues before malignancy. Understanding the role bacteria play, whether it be oncogenesis, tumor p ...

Today's drug discovery strategies require candidate compounds to fail early and cheaply in the discovery stage, rather than late and expensively in the clinical phase. Testing compounds early in physiologically relevant model systems and leveraging rich information from image-based screens are wa ...

At the University of Milano-Bicocca, researchers in the laboratory of Professor Francesco Peri have been investigating the impact of molecules produced by microbiota metabolism, known as postbiotics, on the human host. The team has been using using high-content imaging and AlphaLISA immunoassays ...

Tumor cell function is critically dependent on the local microenvironment and needs to be considered when devising therapeutic strategies to overcome resistance. In this white paper, we discuss how 3D models will help close the gap between what is seen in the preclinical setting and translation i ...