SPA (scintillation proximity assay) beads designed for the capture of [3H]AMP or [3H]GMP on specially prepared yttrium silicate beads.
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SPA Scintillation beads are microspheres containing scintillant which emit light in the blue region of the visible spectrum. As a result, these beads are ideally suited to use with photomultiplier tube (PMT) counters such as the MicroBeta2 or TopCount.
Two types of core SPA Scintillation bead are available - yttrium silicate (YSi) and Polyvinyltoluene (PVT). PVT beads are plastic, larger in size, and stay in suspension longer than the crystalline YSi beads.
Scintillation proximity assay (SPA) is a homogeneous and versatile technology for the rapid and sensitive assay of a wide range of biological processes, including applications using enzyme and receptor targets, radioimmunoassays, and molecular interactions. When 3H, 14C, 33P, and 125I radioisotopes decay, they release β-particles (or Auger electrons, in the case of 125I). The distance these particles travel through an aqueous solution is dependent on the energy of the particle. If a radioactive molecule is held in close enough proximity to a SPA Scintillation Bead or a SPA Imaging Bead, the decay particles stimulate the scintillant within the bead to emit light, which is then detected in a PMT-based scintillation counter or on a CCD-based imager, respectively. However, if the radioactive molecule does not associate with the SPA bead, the decay particles will not have sufficient energy to reach the bead and no light will be emitted. This discrimination of binding by proximity means that no physical separation of bound and free radiochemical is required.
| 自動化兼容 | No |
|---|---|
| 珠型或核心珠型 | YSi |
| 塗層處理 | PDE |
| 檢測方法 | Radiometric |
| 產品品牌名稱 | SPA Scintillation Beads |
| 運輸條件 | 環境 |
| 產品尺寸 | 2 g |
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Many proteins are able to recognize and subsequently bind DNA. These proteins were first identified by their presence in isolated DNA complexes, by their ability to bind DNA in vitro, and by their absolute requirement in many DNA-dependent functions.
Interactions between proteins are a key feature of many biochemical processes, for example cell signalling. In the absence of any enzymatic activity, measurement of protein:protein interactions has presented problems. Scintillation Proximity Assay (SPA) technology permits the direct measurement of b ...
