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PhenoVue 493 Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets.
PhenoVue 493 Lipid stain exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of 爱游戏平台注册登录 's portfolio of cellular imaging reagents, PhenoVue 493 Lipid stain has a maximum excitation wavelength of 493 nm and a maximum emission wavelength of 504 nm,which makes it an alternative to the similar stain BODIPY™ 493/503.
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PhenoVue 493 Lipid stain is a lipophilic fluorescent organic molecule that localizes to polar lipids. It rapidly accumulates in lipid droplets and exhibits enhanced green fluorescence in the presence of neutral lipids like triglycerides and cholesterol esters.
PhenoVue 493 Lipid stain is commonly used for localization and quantification of intracellular lipid droplets which are involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.
| Numbers of Vials Per Unit | 5 |
|---|---|
| Quantity or Volume Per Vial | 2mg (4mM-1.05g/L) |
| Form | Liquid |
| Storage | -20°C |
| Recommended Working Concentration | 5µM (1.31mg/L) |
| Maximum Excitation Wavelength | 493 nm |
| Maximum Emission Wavelength | 504 nm |
| Common Filter Set | FITC |
| Live Cell Staining | Yes |
| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
| Equivalent Number of Microplates | 270-830 x 96-well microplates 230-830 x 384-well microplates 430-1300 x 1536-well microplates |
| Color | Green |
|---|---|
| Detection Method | Fluorescence |
| Filter | FITC |
| Organelle or Cell Compartment | Lipid droplets |
| Product Compatibility | Live and fixed samples |
| Product Brand Name | PhenoVue™ |
| Shipping Condition | Dry Ice |
| Type | Individual Reagent |
| Unit Size | 2 vials |
Cell Painting is a powerful high-content screening method which combines cell and computational biology to unravel cells’ responses and gain a deeper understanding of the effects of chemical and genetic perturbagens.
However, implementation of cell painting is not without its challenges - f ...
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is a product information sheet for PhenoVue 493 Lipid Stain Product.
PhenoVue 493 Lipid Stain
PhenoVue 493 Lipid Stain
HeLa cells stained with PhenoVue 493 Lipid Stain
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green). Imaged on Operetta CLS.
HepG2 cells treated with Oleic Acid-BSA, fixed (PFA), and stained with PhenoVue 493 Lipid Stain (Lipid Droplets, green) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue) (in PBS). Imaged on Operetta CLS.
PhenoVue Cell Painting Kit 10 x 384-wells
List Price : USD1007.0
PhenoVue Hoechst 33342 Nuclear Stain 100mg
List Price : USD108.0
List Price : USD168.0
PhenoVue 578 Mitochondrial Stain
List Price : USD381.0
