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PhenoVue 551 Mitochondrial stain is a fluorescent dye which accumulates in heathly mitochondria of live cells.
PhenoVue 551 Mitochondrial stain exhibits bright orange fluorescence and is validated for use in imaging microscopy and high-content screening applications.
Part of 爱游戏平台注册登录 's portfolio of cellular imaging reagents, PhenoVue 551 Mitochondrial stain has a maximum excitation wavelength of 551 nm and a maximum emission wavelength of 576 nm, which makes it an alternative to the similar stain MitoTracker™ Orange CMTMRos.
false falseFor research use only
PhenoVue 551 Mitochondrial stain is a cationic rosamine based structure. With mitochondrial membrane potential, it accumulates in mitochondria through electrostatic interactions and reacts with thiol moieties forming stable thioether bonds. Therefore PhenoVue 551 Mitochondrial stain is well retained after cell fixation.
Fluorescent mitochondrial stains are commonly used for visualizing and quantifying mitochondria whose dysfunctions, including impaired biogenesis, dynamics or trafficking, are a hallmark of some human diseases, such as neurodegenerative disorders.
PhenoVue 551 Mitochondrial stain can be used to detect mitochondria in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 20 |
|---|---|
| Quantity or Volume Per Vial | 50µg (117nmoles) |
| Form | Dessicated |
| Storage | -20°C |
| Recommended Working Concentration | 100nM (0.04ng/mL) |
| Maximum Excitation Wavelength | 551 nm |
| Maximum Emission Wavelength | 576 nm |
| Common Filter Set | Cy3, RFP |
| Live Cell Staining | Yes |
| Fixed Cell Staining | No |
| Equivalent Number of Microplates | 800-2400 x 96-well microplates 650-2400 x 384-well microplates 1250-3800 x 1536-well microplates |
| 顏色 | Yellow/Orange |
|---|---|
| 檢測方法 | Fluorescence |
| Aequorian親本細胞株 | Cy3 |
| CELL | Mitochondria |
| 型號兼容 | Live and fixed samples |
| 產品品牌名稱 | PhenoVue™ |
| 運輸條件 | 幹冰 |
| Type | Individual Reagent |
| 產品尺寸 | 20 vials |
This is a product information sheet for PhenoVue Mitochondrial Stains.
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
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PhenoVue 551 Mitochondrial Stain
PhenoVue 551 Mitochondrial Stain
HeLa cells stained with PhenoVue 551 Mitochondrial Stain
HeLa cells stained with PhenoVue 551 Mitochondrial Stain
HeLa cells stained with PhenoVue 551 Mitochondrial Stain
U2OS live cells stained with PhenoVue 551 Mitochondrial Stain (mitochondria, orange). Imaged on Opera Phenix Plus.
U2OS live cells stained with PhenoVue 551 Mitochondrial Stain (mitochondria, orange) + PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Opera Phenix Plus.
