PhenoVue 577 Lysosomal stain is a fluorescent dye which accumulates in acidic vesicles such as the lysosomes. The bright red fluorescence associated with PhenoVue 577 Lysosomal stain are validated for use in imaging microscopy and High Content Screening Systems.
false falseLysosomes are abundant organelles which contain more than sixty acidic enzymes. Lysosomes play essential role in macromolecules and organelles break down through the autophagy-lysosomal system. Defective regulation of lysosomal function has been reported in several diseases such as lysosomal storage disorders and neurodegenerative diseases like Alzheimer’s and Parkinson’s. PhenoVue 577 lysosomal stain is partially protonated at neutral pH, and highly protonated at acidic pH. In living cells, PhenoVue 577 lysosomal stain accumulates in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining. PhenoVue 577 lysosomal stain can be used to lysosomes in immunofluorescence, as well as high-content analysis and screening applications.
| Numbers of Vials Per Unit | 20 |
|---|---|
| Quantity or Volume Per Unit | 19.9µg (50nmoles) |
| Form | Solution 1mM DMSO |
| Storage | -16°C or below |
| Recommended Working Concentration | 50 ng/mL |
| Maximum Excitation Wavelength | 577 nm |
| Maximum Emission Wavelength | 590 nm |
| Common Filter Set | RFP |
| Live Cell Staining | Yes |
| Fixed Cell Staining | No |
| Equivalent Number of Microplates | 650 - 2050 x 96-well microplates 550 - 2050 x 384-well microplates 1050 - 3250 x 1536-well microplates |
| 顏色 | Red |
|---|---|
| 檢測方法 | Fluorescence |
| Aequorian親本細胞株 | RFP |
| CELL | Lysosomes |
| 型號兼容 | Live samples only |
| 產品品牌名稱 | PhenoVue™ |
| 運輸條件 | 幹冰 |
| Type | Individual Reagent |
| 產品尺寸 | 20 vials |
In living cells, PhenoVue lysosomal stains accumulate in acidic vesicles such as lysosomes, resulting in bright and specific lysosomal fluorescent staining.
This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
