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PhenoVue Cal-590 AM, Calcium Indicator

PhenoVue Cal-590 AM is a cell permeable fluorescent Ca2+ indicator and is part of Revvity’s portfolio of cellular imaging reagents. PhenoVue Cal-590 AM Ca2+ indicator exhibits orange fluorescence and is validated for use in imaging microscopy and high-content screening applications. It exhibits a maximum excitation wavelength at 574 nm and a maximum emission wavelength of 588 nm.

貨號 CP125901
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For research use only.

詳細信息

Calcium ions are important signaling molecules mediating a broad spectrum of intracellular, intercellular as well as extracellular functions. Ca2+ plays critical physiological functions such as muscle contraction, neuronal excitability, cell migration and cell growth. Intracellular Ca2+ is stored in the endoplasmic reticulum, sarcoplasmic reticulum, and the mitochondria. Upon cell surface receptors stimulation like GPCRs, calcium is released from these internal stores and its intracellular concentration increases from 100 nM to approximately 1 μM.

Intracellular Ca2+ responses have been widely investigated with fluorescent calcium probes which fluorescence signal increases upon Ca2+ binding.

The first-generation of fluorescent calcium indicators, including Fura-2, Fluo-3 and Rhod-2, were developed, followed by a second-generation, such as Fluo-4, with improved properties especially brightness and signal-to-noise ratio. However, the presence of anion transporters expressed at the cell surface may impair the intracellular retention of the fluorescent calcium indicators which are cleared out from cells. Inhibitors of these transporters, such as Probenecid, are commonly used but suffer from limitations such as cytotoxicity, off-targets, or inconsistent calcium flux kinetics. Such limitations can be overcome with third generation of fluorescent calcium indicators including Cal-520, Cal-590 and their corresponding Cal-520, Cal-590 Bright versions which do not require probenecid.

PhenoVue Fluorescent Calcium Indicators, encompassing Fura-2 AM, Fluo-4 AM, Cal-520 AM, Cal-590 AM, Cal-520 AM Bright and Cal-590 AM Bright, enable a myriad of applications as well as multiplexing capability to unravel intracellular calcium mediated functions.

PhenoVue Cal-590 AM is a cell permeable Ca2+ indicator which is metabolized by intracellular esterase and results in a negatively charged fluorescent dye. Consequently, PhenoVue Cal-590 AM exhibits higher sensitivity, significant improved signal-to-noise ratio, reduced leakage and higher intracellular retention compared to other calcium indicators such as Fura-2 AM and Fluo-4 AM.

Features:

  • Numbers of Vials Per Unit: 10
  • Quantity or Volume Per Vial: 50 µg (40 nmoles)
  • Form: Solid
  • Storage: -16 °C
  • Recommended Working Concentration: 5 µM
  • Maximum Excitation Wavelength: 574 nm
  • Maximum Emission Wavelength: 588 nm
  • Common Filter Set: Cy3/TRITC
  • Live Cell Staining: Yes
  • Fixed Cell Staining: No

Equivalent Number of Microplates:

  • 3-10 x 96-well microplates
  • 3-10 x 384-well microplates
  • 4-16 x 1536-well microplates

  

規格

顏色 Orange
檢測方法 Fluorescence
Aequorian親本細胞株 Cy3/TRITC
型號兼容 Live samples only
產品品牌名稱 PhenoVue
運輸條件 幹冰
Type Individual Reagent
產品尺寸 10 vials
資源
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產品手冊

PhenoVue Cellular Imaging Reagents Brochure

This brochure describes Revvity's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.

PDF 3 MB
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