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PhenoVue™ DAPI nuclear stain is a cell-impermeable organic molecule that binds to DNA and is commonly used to stain cell's nucleus.
Part of 爱游戏平台注册登录 ’s portfolio of cellular imaging reagents, the PhenoVue DAPI nuclear stain has a maximum excitation wavelength of 348nm and a maximum emission wavelength of 457nm.
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PhenoVue DAPI nuclear stain is a cell-impermeable organic molecule that binds preferentially to adenine-thymine (A-T) regions of minor groove of DNA. When excited by ultraviolet light, DAPI exhibits strong fluorescence at 455 nm. It is commonly used to stain cells’ nuclear compartment.
It can be used in indirect immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Numbers of Vials Per Unit | 1 |
---|---|
Quantity or Volume Per Unit | 1 mg (1mg/mL - 2.85mM) |
Form | Liquid |
Storage | -20°C |
Recommended Working Concentration | 1µg/mL (10nM) |
Maximum Excitation Wavelength | 358 nm |
Maximum Emission Wavelength | 457 nm |
Common Filter Set | DAPI |
Live Cell Staining | usually not, except at high concentrations |
Fixed Cell Staining | Yes |
Equivalent Number of Microplates |
350-1040 x 96-well microplates |
Color | Blue |
---|---|
Detection Method | Fluorescence |
Filter | DAPI |
Organelle or Cell Compartment | Nucleus |
Product Compatibility | Fixed samples only |
Product Brand Name | PhenoVue™ |
Shipping Condition | Ambient |
Type | Individual Reagent |
Unit Size | 1 vial |
This brochure describes 爱游戏平台注册登录 's PhenoVue cellular imaging reagents plus microplates, Imaging instruments, imaging and analysis software and automation.
This is the product information sheet for PhenoVue DAPI Nuclear Stain.